Hello, I've read the help page, and I believe my three data frames meet all the requirements, but the creation of the TranscriptDb results in an error message.
My code example is : library(GenomicFeatures) download.file("http://129.94.136.7/file_dump/dario/dataFramesForTrDB.Rdata", "./3DF.Rdata") # 2.2 MB load("3DF.Rdata") # transcripts, splicings, genes. db <- makeTranscriptDb(transcripts, splicings, genes) Error in sqliteExecStatement(con, statement, bind.data) : RS-DBI driver: (RS_SQLite_exec: could not execute: constraint failed) Session info is : R version 2.13.0 (2011-04-13) Platform: x86_64-unknown-linux-gnu (64-bit) ... ... other attached packages: [1] GenomicFeatures_1.4.1 GenomicRanges_1.4.5 IRanges_1.10.4 loaded via a namespace (and not attached): [1] Biobase_2.11.10 biomaRt_2.7.2 Biostrings_2.19.18 [4] BSgenome_1.19.6 DBI_0.2-5 RCurl_1.6-1 [7] RSQLite_0.9-4 rtracklayer_1.11.12 XML_3.4-0 -------------------------------------- Dario Strbenac Research Assistant Cancer Epigenetics Garvan Institute of Medical Research Darlinghurst NSW 2010 Australia _______________________________________________ Bioc-sig-sequencing mailing list Bioc-sig-sequencing@r-project.org https://stat.ethz.ch/mailman/listinfo/bioc-sig-sequencing