dear members: i have a question, that is i remove 5' and 3' N, and do statistics on reads with N in the middle then, i removed reads with N less than the cutoff but those remainding reads still contain N so i must use their id to retrieve original reads, See #1 so what can i do?
part of coding seqsWithoutNend <-trimLRPatterns(Rpattern = letter_subject, Lpattern = letter_subject,subject = injectedseqs) nCount<-alphabetFrequency(seqsWithoutNend)[,"N"] nDist<- table(nCount) cutoff=2 filter1 <- nFilter(threshold=cutoff) qualifiedReads <- reads[filter1(seqsWithoutNend)] reads[id(qualifiedReads)] #1 [[alternative HTML version deleted]] _______________________________________________ Bioc-sig-sequencing mailing list Bioc-sig-sequencing@r-project.org https://stat.ethz.ch/mailman/listinfo/bioc-sig-sequencing