From: "Miao, Lingling (NIH/NIAMS) [F]" <[email protected]> Reply-To: "Miao, Lingling (NIH/NIAMS) [F]" <[email protected]> Date: Thursday, September 6, 2018 at 7:26 PM To: List SINGLECELLGENOMICS-L <[email protected]> Subject: Partek Single Cell RNA-Seq Data Analysis Seminar
Single Cell RNA-Seq Data Analysis Seminar When: 1:00PM - 2:00PM, Wednesday, September 19th. Where: Conference Room 1327, Building 50 (50/1327), NIH Campus The Single Cell RNA-Seq data analysis pipeline in Partek Flow simplifies Single Cell RNA-Seq analysis, even for novices! During this seminar session, Partek Scientist will show you how to analyze a Single Cell RNA-Seq data set with multiple biological replicates and detect genes that are differentially expressed between cell populations across sample groups in an intuitive, interactive and powerful way. Agenda: Partek Single Cell RNA-Seq Data Analysis Solution Overview Live Demo * Import count matrix text file or fastq files and upstream analysis pipeline on 10X prep kit if users are interested * Filter cells using interactive QA/QC charts * Filter low expressed genes * Normalize raw count * Visualize cell populations using the interactive 3D t-SNE plot * Overlay gene expression and pathway signatures on the 3D t-SNE plot * Select and classify cells on the 3D t-SNE plot * Detect differentially expressed genes between sub populations * Filter a gene list * Identify enriched KEGG pathway and/or GO terms * Visualize cell-level results using heat maps, volcano plots, and violin plots Q&A ________________________________ To unsubscribe from the SINGLECELLGENOMICS-L list, click the following link: http://list.nih.gov/cgi-bin/wa.exe?SUBED1=SINGLECELLGENOMICS-L&A=1
