Well - Amore does it easily, providing the rotation function works.
Not sure it is properly documented or available in the GUI though..
You need to INPUT at the sortfun stage LABI FP=F PHI=phase W=Fom
That requires if you are using the GUI that you use the option
Run and View com file
then edit the LABIN line
Then the rotation function runs as usual, and you need to ask at the
translation stage, not for Crowther blow, but phased tranlation with
external phases
You search for the 2nd molecule in the standard way.
Run translation function
Add known solutions
etc etc
Molrep - you can choose to use the phases
it is one of the start options. It uses the phases for the rotation
function as well; sometimes more effective - sometimes less.
Or you can use ffear - give as your search molecule your MR solution -
pref after a bit of refinement, then ffear should find both.
Eleanor
ZhiYi Wei wrote:
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hi, all
I want to do a phased MR, but I can not figure out how to do it.
Some backgrounds:
the protein containing two subunits;
a poor SAD-phasing map;
got a correct MR of one subunit.
A phased MR can be used to solve the position of the other subunit. The question
is that how to fix the correct subunit position in a phased MR, which program
can
do that?
Thanks alot.
------------------------------
Zhiyi Wei
---------------------------------------------
Institute of Biophysics, Chinese Academy of Sciences
15 Datun Road, Chaoyang District
Beijing, P.R.China, 100101
---------------------------------------------
School of Life Sciences,
University of Science & Technology of China,
Hefei, Anhui, P.R.China, 230026
Tel:86-551-3607334
Fax:86-551-3607154
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