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Hi, looking at my example in the documentation, the 2nd table is the one you need to check out first (the 1st table is just an echo of the input RF peak positions). It gives a list of pairs of input RF peaks (incl symmetry-related) with the corresponding polar angles and SRF value (sorted in descending order of the latter). The columns labelled #Peak reference the peak nos in table 1. If you're expecting only a 2-fold, then you should specify chi=180 in the input (as in the example) and the program will give you only 2-fold solutions. Then all pairs of peaks in table 2 are potential solutions: you may have to try them all, but usually it's obvious which is the correct RF pair to use. Table 3 gives the inter-vector angles between putative rotation axes. However if you're looking for just one NCS 2-fold then table 3 is not going to tell you anything you didn't know already. It's useful if say you're expecting 3 2-folds in 222 NCS (as in the example) and you want to select sets of rotation axes that are 90 deg apart. AFAIK Phaser uses the same Eulerian angle convention as ALMN & AMoRe (i.e. the 'Crowther' convention), but Randy will no doubt confirm that. -- Ian > -----Original Message----- > From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On > Behalf Of A Hibbert > Sent: 14 November 2005 19:16 > To: [email protected] > Subject: [ccp4bb]: PHASER/RFCORR/Self-Rot. > > *** For details on how to be removed from this list visit the *** > *** CCP4 home page http://www.ccp4.ac.uk *** > > > Thanks for the e-mails regarding application of self-rotation > function > solutions to PHASER. > > I have tried out RFCORR, but don't understand how to > interpret the output. > According to the documentation the 3rd/last table should show > what I'm > looking for (i.e., application of cross rotation functions x > and would give > the best agreement with self rotation function z. [I have two > molecules per > ASU, and can see a clear peak @ k=180 in the SRF]). However, > could someone > please explain how to correctly analyze and filter out the > best results. > > P.S: Am I right in thinking that I can put PHASER x-rotation > solutions > straight into RFCORR (i.e., does PHASER use the same > definition of Eulerian > angles as RFCORR)? > > Thanks! > > _________________________________________________________________ > Don't just search. Find. Check out the new MSN Search! > http://search.msn.click-url.com/go/onm00200636ave/direct/01/ > > > Disclaimer This communication is confidential and may contain privileged information intended solely for the named addressee(s). It may not be used or disclosed except for the purpose for which it has been sent. If you are not the intended recipient you must not review, use, disclose, copy, distribute or take any action in reliance upon it. If you have received this communication in error, please notify Astex Therapeutics Ltd by emailing [EMAIL PROTECTED] and destroy all copies of the message and any attached documents. Astex Therapeutics Ltd monitors, controls and protects all its messaging traffic in compliance with its corporate email policy. The Company accepts no liability or responsibility for any onward transmission or use of emails and attachments having left the Astex Therapeutics domain. Unless expressly stated, opinions in this message are those of the individual sender and not of Astex Therapeutics Ltd. The recipient should check this email and any attachments for the presence of computer viruses. Astex Therapeutics Ltd accepts no liability for damage caused by any virus transmitted by this email. E-mail is susceptible to data corruption, interception, unauthorized amendment, and tampering, Astex Therapeutics Ltd only send and receive e-mails on the basis that the Company is not liable for any such alteration or any consequences thereof.
