*** For details on how to be removed from this list visit the *** *** CCP4 home page http://www.ccp4.ac.uk ***
On Thu, 2005-12-01 at 16:43 -0500, [EMAIL PROTECTED] wrote: > *** For details on how to be removed from this list visit the *** > *** CCP4 home page http://www.ccp4.ac.uk *** > > > Apologies for continuing to top-post here... > > If the cleavage would only remove 28 amino acids from a 500 amino acid > chain, it could easily be missed by SDS-PAGE, although mass spec would > catch it. However, I don't think you can get enough sample from a > dissolved crystal to run mass spec. Absolutely, yes you can. What is the low resolution cut-off on the data used in refinement? A lack of low-res data (cut off of 6 - 10 A) can cause map errors such as apparent chain breaks. Cheers, -- ======================================================================= I've gained no wisdom, no insight, no mellowing. I would make all the same mistakes again, today. - Woody Allen ======================================================================= David J. Schuller modern man in a post-modern world MacCHESS, Cornell University [EMAIL PROTECTED]
