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I'm grateful to Dan for this citation! I thought of replying to the
initial request.
I think that there is a reference in this chapter to a paper where I
got the idea, perhaps from Timasheff.
I have to add that the idea captivated me, together with the notion
that other additives could serve specific kinds of purposes for
different protein crystallization problems. Unfortunately, despite
some
considerable effort to demonstrate an effect (either positive or
negative) of arginine on several systems I was interested in
crystallizing came to nought. I do recollect that a crystal structure
of Grb2 from Arnaud Ducruix's lab may have benefitted from the use of
arginine in the crystallization mix. However, that example was
anecdotal and did not venture into the arena of experimental proof.
Thus, arginine joins an (impossibly) long list of possibilities for
which there are arguments, but little data.
On Jan 24, 2006, at 1:07 PM, Daniel Anderson wrote:
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Yes, there's a long-ish history of using arginine as crystallization
additive. See for examples:
Charlie Carter's Chapter 4 (pages 75-120) in "Crystallization of
Nucleic
Acids and Proteins", second edition, Ducruix, Giege, editors.
DeLucas, et al J.Struct. Biol. (2003) 142, 188-206.
There are likely others.
-Dan Anderson
On Tue, 24 Jan 2006, Stein, ND (Norman) wrote:
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Hi all,
I was wondering if anyone's had any experience using arginine as a
crystallization additive, and generally as a useful chemical to
prevent
aggregation of globular, but sticky proteins. The paper that set us
thinking
about is: Journal of Chromatography A, 1094 (2005) 49 - 55.
Thanks in advance for any tip and advice,
Luca
#########################################
Luca Pellegrini
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Biochemistry Department
University of Cambridge
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### a low-throughput crystallographer ###
**********UNCrystallographers NOTE new website url******************
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**********UNCrystallographers NOTE new website url******************
[EMAIL PROTECTED]
http://xtal.med.unc.edu/CARTER/Welcome.html
Department of Biochemistry and Biophysics CB 7260
UNC Chapel Hill, Chapel Hill, NC 27599-7260
Tel: 919 966-3263
FAX 919 966-2852
