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Hi Florian, depending on how your data has been scaled it's possible that the default contour level settings in POLARRFN (keyword PLOT) are not suitable. Did you try reducing the contour start level and interval? I just tried some test runs of the versions of POLARRFN in CCP4 5.0.2 & 6.0 and they both seem to be OK (and give essentially the same result). Cheers -- Ian > -----Original Message----- > From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On > Behalf Of Florian Schmitzberger > Sent: Tuesday, April 04, 2006 1:54 PM > To: [email protected] > Subject: [ccp4bb]: self-rotation in POLARRFN > > *** For details on how to be removed from this list visit the *** > *** CCP4 home page http://www.ccp4.ac.uk *** > > > Dear All, > > I apologize if this issue has been raised before, but I could not > find a posting about it. > > I have been trying to calculate Patterson self-rotation functions in > POLARRFN in CCP4i (CCP4 version 5.99.5; Mac OSX 10.4.5.) using > scaled and merged reflection files from either XDS/XSCALE or SCALA. > Everything appears to work fine (clear 2-fold axis in P2 > space group, > indicating C222) when using the merged, scaled, and converted > reflections from XDS, with the default parameters in POLARRFN. > However, when I use the truncated structure factors from SCALA (from > data processed in MOSFLM) I don't see any peaks in the self-rotation > maps and all kappa-angle slices are completely blank. > > I have played around with integration radius, resolution etc. and > tried to run it from the command line, but to no effect. I suppose I > am missing something obvious, but can't seem to be able to figure it > out. > > Has anyone got an idea what the problem may be? > > Thank you very much! > > Regards, > > Florian > -------------------------------------------- > Florian Schmitzberger > Medical Biochemistry and Biophysics > Karolinska Institute > Scheeles vaeg 2 > SE-171 77 Stockholm, Sweden > Tel: +46-8-524-86875 > > > > > Disclaimer This communication is confidential and may contain privileged information intended solely for the named addressee(s). It may not be used or disclosed except for the purpose for which it has been sent. If you are not the intended recipient you must not review, use, disclose, copy, distribute or take any action in reliance upon it. If you have received this communication in error, please notify Astex Therapeutics Ltd by emailing [EMAIL PROTECTED] and destroy all copies of the message and any attached documents. Astex Therapeutics Ltd monitors, controls and protects all its messaging traffic in compliance with its corporate email policy. The Company accepts no liability or responsibility for any onward transmission or use of emails and attachments having left the Astex Therapeutics domain. Unless expressly stated, opinions in this message are those of the individual sender and not of Astex Therapeutics Ltd. The recipient should check this email and any attachments for the presence of computer viruses. Astex Therapeutics Ltd accepts no liability for damage caused by any virus transmitted by this email. E-mail is susceptible to data corruption, interception, unauthorized amendment, and tampering, Astex Therapeutics Ltd only send and receive e-mails on the basis that the Company is not liable for any such alteration or any consequences thereof.
