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Dear Vincenzo,
Very often it works not to use any cryoprotectant at all, and just to
dip the crystal with its loop briefly into paraffine oil. I used the one
which was sold some time ago as part of the panjelly kit by Jena
Biosciences and had a lot of very positive experiences, as long as the
crystals have a reasonable size (not too small and not too big)and a
compact shape.
Greetings
Wim
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Wim Burmeister
Professeur, Membre de l'Institut Universitaire de France
Laboratoire de Virologie Moleculaire et Structurale FRE 2854 CNRS-UJF
c/o EMBL, 6 rue Jules Horowitz
B.P. 181, F-38042 Grenoble Cedex 9 FRANCE
E-mail: [EMAIL PROTECTED]
Tel: +33 (0) 476 20 72 82 Fax: +33 (0) 476 20 71 99
http://www2.ujf-grenoble.fr/pharmacie/laboratoires/gdrviro
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