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Dear Vincenzo,

Very often it works not to use any cryoprotectant at all, and just to dip the crystal with its loop briefly into paraffine oil. I used the one which was sold some time ago as part of the panjelly kit by Jena Biosciences and had a lot of very positive experiences, as long as the crystals have a reasonable size (not too small and not too big)and a compact shape.

Greetings

                                                                        Wim

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Wim Burmeister
Professeur, Membre de l'Institut Universitaire de France
Laboratoire de Virologie Moleculaire et Structurale FRE 2854 CNRS-UJF
c/o EMBL, 6 rue Jules Horowitz
B.P. 181, F-38042 Grenoble Cedex 9          FRANCE
E-mail: [EMAIL PROTECTED]
Tel:    +33 (0) 476 20 72 82       Fax: +33 (0) 476 20 71 99
http://www2.ujf-grenoble.fr/pharmacie/laboratoires/gdrviro
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