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Hi Dirk, we have been experiencing similar problems as well and we are the moment testing the idea of trying intermediate volumes as we go from the 200-300 nL droplets to 1-2 uL, for example 0.5uL +0.5 uL in the first instance, while keeping the same type of plate and the same droplet:reservoir volume ratio. Best regards Savvas -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Dirk Kostrewa Sent: dinsdag 11 april 2006 9:31 To: CCP4BB Subject: Re: [ccp4bb]: Protein crystallization *** For details on how to be removed from this list visit the *** *** CCP4 home page http://www.ccp4.ac.uk *** Dear CCP4ers, Nat Echols wrote: ... > I would definitely say that reproducing the crystals in a more > standard > format (i.e. 24-well sitting/hanging drop trays) doesn't always work as > expected, but I've had this problem regardless of screen format. (I > suspect as more and more people use robots this will continue to be an > issue.) ... yes, we have quite often problems now with upscaling an initial crystallization condition from our robot 96 well plates with typically 200-500 + 200-500 nl drop size to conventional 24 wells with 2-3 + 2-3 ul drop size. It's clear, that the geometry is somewhat different, and presumably the smaller drops mix faster, but currently, I have no idea what the major obstacle for upscaling is. Does anybody of you have a clue and, maybe, a solution or workaround? Best regards, Dirk. -- **************************************** Dirk Kostrewa Paul Scherrer Institut Biomolecular Research, OFLC/110 CH-5232 Villigen PSI, Switzerland Phone: +41-56-310-4722 Fax: +41-56-310-5288 E-mail: [EMAIL PROTECTED] http://sb.web.psi.ch ****************************************
