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Two things to try: 1. EDEN using phase perturbation and an explicitly generated solvent mask 2. A composite-omit map in CNS (which by default will include a solvent term as well). Phil Jeffrey wrote: > *** For details on how to be removed from this list visit the *** > *** CCP4 home page http://www.ccp4.ac.uk *** > > > Something I have never found to work to my satisfaction has been solvent > flattening after molecular replacement where the model is somewhat > partial (i.e. more than 20% of the new structure is not modeled by the > MR solution). In a case I was trying out for someone else a few months > back I used DM with a variety of automatic and more manually-generated > solvent masks, and this produced no significant gain in the phases to my > eyes. So I am left with the suspicion that I'm not using the best > methods. > > Does anyone have any advice ? - I've found Coulombe and Cygler's 1997 > paper (Acta Cryst D53, 426-433) - but I imagine that there is other > information out there that an hour's Googling has yet to turn up. > > Thanks > Phil Jeffrey > Princeton, NJ >
