*** For details on how to be removed from this list visit the ***
*** CCP4 home page http://www.ccp4.ac.uk ***
shivesh kumar wrote:
Dear All,
We are trying to solve the structure of a 15 kDa protein with p63
space group.We are able to trace only 50% of the molecule and R and
free_R is 25.7 and 28.2,respectively.We could not find any extra
electron density in the unit cell , and we confirmed that we have
crystallized full molecule.Can anybody help in this regard.Can we
publish this 50% structure.
Thanx in advance.
Shivesh
Hi,
did you confirm the dissolved crystal or the protein solution (?) via
SDS PAGE or mass spectrometry ? Are you missing ~30 residues of the
C-term and roughly the same at the N-term ? Did you use a tag at one of
the ends ? Cleaved afterwards ?
If you run DisEMBL on your sequence are any of those regions predicted
to be disordered ?
If you have 2.6-2.8 A data, then the R values are OK, but I suspect you
have better resolution than that ? How many waters have you added to
your structure, are they all waters or could they be part of the missing
backbone ?
Did you use TLS-refinement ? What about CNS composite omit maps with SA
? Have you tried GraphENT ?
Just a few thoughts, good luck !
Jürgen
--
Jürgen Bosch
Howard Hughes Medical Institute and
University of Washington
Dept. of Biochemistry, K-426
1705 NE Pacific Street
Seattle, WA 98195
Box 357742
Phone: +1-206-616-4510
FAX: +1-206-685-7002
