Re: [ccp4bb]: Fitting a large fragment to ED

[Debanu Das]

Hi,    Phased Translation Function would be the thing to try. This will allow you to input the experimental phases and the fit the expt. density onto the density calculated from the model while searching over orientation/rotation  space  if you don't already know the orientation solution. You could try BRUTEPTF at http://russel.bioc.aecom.yu.edu/server/NYSGRC.html A description is availabe in a recent paper with a different approach to do PTF. Acta Crystallogr D Biol Crystallogr. 2005 Mar;61(Pt 3):285-93. Epub 2005 Feb 24. Related Articles, Links You could also look at using ESSENS in the Uppsala SF. Regards, Debanu. -- Debanu Das, PhD, University of California, Berkeley. Derek Logan wrote: Dear all, Short of the painful process of moving it by hand, what is the currently most efficient way to place a large protein fragment (say 350 amino acids) in an experimentally phased electron density map? The resolution is 2.7Å and we can see some secondary structure elements in the map. Thanks Derek -- Derek Logan             tel: +46 46 222 1443 Associate professor     fax: +46 46 222 4692 Molecular Biophysics         Lund University Box 124, Lund, Sweden