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One of my coworkers has had success with hydrophobic peptides by
treating them like organic molecules; batch crystallization from organic
phases has worked well. See eg De Simone, Giuseppina; Lombardi, Angela;
Galdiero, Stefania; Nastri, Flavia; Di Costanzo, Luigi; Gohda, Shin;
Sano, Atsuiro; Yamada, Takashi; Pavone, Vincenzo. The crystal structure
of a Dcp-containing peptide. Biopolymers (2000), 53(2), 182-188.
good luck
kmj
ferrarod wrote:
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Hello everyone,
Sorry for the off topic question. I was wondering if anyone had any ideas or
suggestions on protein crystallization of a small peptide. It is 46 amino
acids and it is very hydrophobic. It is not soluble in buffered water, but
it is soluble in 70% acetonitrile and in detergent. We have tried
traditional sparse matrix screens, but we get precipitate even at low
concentrations (i.e. 3 mg/ml). Any advice or suggestions would be
appreciated.
Cheers,
Dan
