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Hello All, I'm attempting to co-crystallize a dimeric transcription factor bound to DNA and see skin formation and microcrystals at even the lowest concentration of precipitant that I have tried. The complex crystallizes (I believe) on storage at 4 deg, but these crystals do not diffract. I have additionally tried using increased concentrations of reducing agents and also covalent modifications of Cys residues and still see skin formation. After long term storage, larger crystals grow after a few months storage, but are leaf shaped and growing in clumps. I have not checked the diffraction limits of these crystals as yet (no access to local x-ray source). Any advice as to how to control the seeding events and/or skin formation would be appreciated. Thanks! Fi Fiona Whelan Whitelaw Lab School of Molecular and Biomedical Science (Biochemistry) The University of Adelaide Phone: 8303 4806
