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Can someone do something about this spam? My mail box is flooded with 100s of spam mails from Michael K Swan ES ----- Original Message ----- From: Michael K Swan <[EMAIL PROTECTED]> Date: Saturday, December 2, 2006 6:58 pm Subject: [ccp4bb]: HKL2000 processing. To: ccp4bb@dl.ac.uk > Hi All, > I have a question about processing in HKL2000. > I have a dataset which looks like it will go out to 2.5A but the > problem > is that when I have integrated the 360 frames (180degrees) to 2.4A > I get > a large number of rejects on scaling which exceeds the 50000 limit. > So > is there any way to increase this limit or another method of > reducing > the number of rejects? > I can get scaling to work if I either cut the resolution down to > 3.0 or > lower but alternatively if I exclude alot of frames and leave say > 90 > then the rejects are below 50000 and the scaling produces data that > looks good to at least 2.6-2.5A according to the I/sigma and an > Rmerge > <0.5 in the final shell, however the completeness suffers due to > the low > number of frames. > I should also say that I am suspicious that one of the reasons for > all > the rejects might be that in the centre of the pattern the spots > are of > a good radial shape but towards the edge they become more > elongated. I > tried using the elliptical spot feature of HKL2000 but this doesn't > seem > to be ideal as the spots are not always elongated in the same > direction.Any thoughts or suggestions would be much appreciated, > > Mike Swan. > From: Dr. E. Subramanian Emeritus Professor of Biophysics University of Madras
