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Hi Patrick, Recently in this forum a paper was suggested for different reason, but I think it will be applicable for your case as well. E. Saridakis, N.E. Chayen Protein Sci. 2000 9: 755-757. P.S. If you can grow crystals of nano-flowers in your nano-vase you may be able to market it around Valentine's Day. Vaheh Oganesyan, PhD Scientist II MedImmune, Inc. One MedImmune Way Gaithersburg, MD 20878 USA Phone: (301)398-5851 www.medimmune.com -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] Behalf Of Patrick Loll Sent: Tuesday, December 05, 2006 1:32 PM To: ccp4bb@dl.ac.uk Subject: [ccp4bb]: reducing crystal imperfections *** For details on how to be removed from this list visit the *** *** CCP4 home page http://www.ccp4.ac.uk *** We have a system where we can produce rod-shaped crystals that show a classic depletion imperfection--the rods are essentially hollow for most of their length, suggesting that growth is proceeding in this direction more rapidly than fresh protein can diffuse into the area (picture a long hexagonal rod with a perfect conical hole bored into one end, extending for most of the rod's length). Simple-minded optimization experiments (e.g., [PEG] vs. [salt] vs. pH) have clear-cut effects on nucleation, but don't seem to alter crystal shape, which I interpret as meaning that growth occurs rapidly once nucleation has taken place. What are peoples' thoughts on improving the morphology? We're currently exploring whether additives can selectively poison growth along the long axis, but I'd be curious to hear other ideas. For example, might gels help, or are they mainly effective in controlling nucleation? They ARE pretty cool looking. I wonder if there's a market for nanovases... Pat ------------------------------------------------------------------------ --------------- Patrick J. Loll, Ph. D. (215) 762-7706 Associate Professor FAX: (215) 762-4452 Department of Biochemistry & Molecular Biology Director, Biochemistry Graduate Program Drexel University College of Medicine Room 10-102 New College Building 245 N. 15th St., Mailstop 497 Philadelphia, PA 19102-1192 USA [EMAIL PROTECTED]
