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Hello All,
I wonder if anyone has experience with making semet substituted
proteins in insect cells.
Specifically, we have been making native protein using sf21 cells
grown on Sf900 II media from invitrogen.
I see that invitrogen also carries Sf900 II media that lacks
methionine and cystine. My experience with other media preparations
(I've used Excel 405 before) was that for best results
I had to special order it without methionine and also without
yeastolate. I have been unable to find out from Invitrogen whether
Sf900 II contains yeastolate (ingredients are secret; also, I was
told that special ordering would cost 3K in USD
for 6L, and I am still coughing and not sure about coughing up 3K).
so I wonder if anyone has made semet substituted protein using sf900
II without met and cystine, as offered by Invitrogen--and whether
semet substitution was sufficiently high for SAD/MAD?
Thanks so much,
Karin
