To understand this one at least needs the P1 unit cell..
Operators 3 4 and 5 are almost the same rotation.. It is unlikely that
they are all 2 folds?
Eleanor
Daniel Adams wrote:
Hi bb,
I am working on P1 dataset, SAD phasing resolution is 3.5 and trying
to build model. However I am facing difficulties in improving map
using NCS (in DM and resolve ).
Mathews content and SRF indicate that there are 8 molecules and most
likely two tetramer is ASU. There is also pseudotranslation
(orthogonal vector is: 11.7 0 0)
Biochemically it shows that there is a dimer as basic unit i.e. two
dimer in one tetramer.
Using Se HA site, findncs indicates following 2 folds (in polar angle):
1. 23.5 162 180, translation_orthogonal: 0.14 22.7 -3.13
2. 9.2 88.14 180,translation_orthogonal: -71.5 -0.25 0.41
3. 95.4 180 180, translation_orthogonal: -0.07 -68.1 -2.67
4. 90 179 180, trnaslation_orthogonal: 0.29 22.6 -2.8
5. 90 1.3 180, translation_orthogonal: - 0.5 22.7 -2.8
However when I use above 2 folds as NCS, map doesn't improve at all...
My struggle is how can I use this combination of NCS together in DM or
resolve ?
Is there something I am missing ?
Thanks in advance
Daniel
