Just to add that in one case we were able to get suitable crystals after using a protein concentration of as low as 1.5 mg/ml (kapetaniou et al. Acta Cryst. 2005, F61, 479-481) and microdialysis to slowly remove the excess of salt. Lower concentrations (0.50-0.75 mg/ml) could also produce crystals but they were too small.
Tassos Papageorgiou Quoting Patrick Shaw Stewart <[EMAIL PROTECTED]>: > Ronaldo > > I have a database of crystallization conditions extracted from the > PDB. I was able to parse the data to extract the concentration of > protein in around 900 cases. > > The lowest 9 protein concentrations were as follows: > > PDB ID TYPE DATE Protein conc, mg/ml > 1EYM ISOMERASE. 07/05/2000 0.75 > 1ADQ COMPLEX (IMMUNOGLOBULIN/AUTOANTIGEN) 18/02/1997 1 > 1W5G FOUR HELIX BUNDLE. 06/08/2004 1 > 1UU4 HYDROLASE. 15/12/2003 1 > 1W2U HYDROLASE. 08/07/2004 1 > 1DXP SERINE PROTEASE. 13/01/2000 1 > 1DY8 SERINE PROTEASE. 18/01/2000 1 > 1DY9 SERINE PROTEASE. 31/01/2000 1 > 1DG6 APOPTOSIS. 23/11/1999 1.2 > > The maximum protein conc was 200 mg/ml (1BLF) > > My database goes up to October 2004. Janet Newman and Tom Peat have > done this job far more conscientiously and could probably give you > better and more up-to-date data. > > Patrick > > > On 2/23/07, Ronaldo Alves Pinto Nagem <[EMAIL PROTECTED]> wrote: > > > > > > Dear all, > > > > As protein crystallization is the main topic of the day may I include > > another question? > > > > What was the minimun protein concentration reported with success in > > crystallization trials? > > > > I ask that because the protein I am trying to crystallize is much less > > soluble than the one mentioned in the lasts emails. > > > > Thanks > > > > Ronaldo. > > > -- A.C.(Tassos) Papageorgiou, PhD phone: +358 2 333 8012 (office) Senior Scientist, Group leader fax: +358 2 333 8000 Turku Centre for Biotechnology E-mail: [EMAIL PROTECTED] BioCity, Turku URL: http://www.btk.utu.fi/~apapageo FIN-20521, Finland
