Just a suggestion. To avoid the glass cover slips, one can use the hanging drop trays from Qiagen (formerly Nextal). No risk of tearing gloves and no grease to mess with either. Crystals can be set up in any glovebox and then the tray will hold the seal using an 0-ring. Nice to be able tomanipulate the crystals in case you have more than one per drop that you want to freeze.
Tim Wood > Dear Mathews, > > we were successful in crystallizing two ferredoxins under strict anaerobic > conditions using the much cheaper solution of a glove bag and filling it > with argon. I guess that in both cases (box and bag) the thickness of the > gloves is a problem especially if you are dealing with cover slides. The > glove bag occupies also much less space however its major problem was the > very bad visualization of the inner space and finally we added a plexiglas > window on the bag. Needless to say how essential is not to forget anything > outside the bag/box before starting the crystallization set up. > Finally you can also consider the possibility to use a crystallization > robot. In some of them it is possible to create anaerobic conditions using > argon. > > good luck > Nikos > > > ************************************* > Nikos Pinotsis, PhD > EMBL-Hamburg, c/o DESY > Notkestr. 85, Geb. 25A > 22603 Hamburg, Germany > > Phone : +49 40 89902144 > Fax : +49 40 89902149 > e-mail : [EMAIL PROTECTED] > ************************************* > > ----- Original Message ----- > From: "Mathews, Irimpan" <[EMAIL PROTECTED]> > To: <CCP4BB@JISCMAIL.AC.UK> > Sent: Friday, 23 February, 2007 3:03 AM > Subject: [ccp4bb] Question about glove boxes for protein crystallization > > > Dear Friends, > > Sorry for the non CCP4 question. We are planning to purchase a small glove > box to setup crystallization trays under anaerobic conditions. If you have > used glove boxes for crystallization, would you please give me some idea? > > We are thinking of getting the 815 series from Plas-labs (link below). > > http://www.plas-labs.com/ > > Thank you very much, > Mathews > > Ps: If others are interested, I will post a summary. >
