Hi all,
Thanks for those who replied so far. I can see that the
solubility issue is not that problematic for the crystallization work
(as Kendall mentioned).
I recall that some people on the board reported in a different
thread that they tried the solid powder in the crystallization drop
and it worked!
The biggest concern is with the planned activity assay! For that,
I think I need to get a clear solution with known concentration of
the compound to be tested.
P.S. Just a correction, I meant we have 10 mg of each compounds.
The compounds received as powder.
At 11:28 AM 2/28/2007, you wrote:
Dear all,
I have a small library of In-silico screened compounds to test
for activity and for crystallization trials with our protein of interest.
We only have about 10 mg/ml of each compound. As there is no
available experimental information about solubility of these
compounds, I have no choice but to try different solvents.
The first solvent to try will be DMSO (100%) to make the highest
stock concentration of each compound. My question to those who
passed through similar experience is:
Assuming some of the compounds turned to be insoluble in DMSO,
which is possible, how to completely recover the compounds from
DMSO before trying another solvent.
Will spinning and leaving the tube open over the bench be enough
to get rid of the solvent or what people usually do in that case?
What is the recommended solvent to try next?
Is there a standard protocol to follow for the case we have??
thanks in advance for those who are willing to share their experience.
regards,
Ibrahim
Ibrahim M.Moustafa, Ph.D.
Pennsylvania State University
Biochemistry & Molecular Biology Dept.
201 Althouse Lab.
University Park, PA16802
Tel (814) 863 8703
Fax (814) 865 7927
Ibrahim M.Moustafa, Ph.D.
Pennsylvania State University
Biochemistry & Molecular Biology Dept.
201 Althouse Lab.
University Park, PA16802
Tel (814) 863 8703
Fax (814) 865 7927
