Hai All, Thanks for all the good suggestions. These are the ideas I received . Growing crystals at different temperature, 4 C, 15C,21C and 10C. Add glycerol or ethylene glycol - set a screen with your condition as basis, and % EG/glycerol across - from 1% to 15% in suitable increments Find additives that produce no crystals, set up tray(s) with those, then seed with microcrystals. Reduce protein concentration. Add gelling agents to the set-up Also one member suggested that increasing protein concentration and reducing the PEG concentration can increase the crystal size. Microseeding. Cross-seeding a complete screen with microcrystals. Crystallization in sitting drops under oil. Jobichen Chacko.
