Hi,
In my opinion this is clearly a twinned crystal of some sort of a small molecule. If you have a small ice crystal on top of a completely non-diffractive protein crystal, sometimes the pattern may also look like this - but I would bet this is just 'salt'. Artem _____ From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Noinaj Sent: Wednesday, April 18, 2007 7:19 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] protein or salt? Hi, i collected a diffraction pattern today of a protein crystal, hoping to answer the question, is it a protein or salt? The crystals appear as typical proteins crystals (from my experience), forming both plates and rods, easily crushed into millions of tiny crystals, and are bouyant. unfortunately, i collected a diffraction pattern that is a bit confusing. you can see the image at the following URL: http://www.noinaj.com/temp/screen1.jpg while i don't see very low resolutions spots, i do see spots lower than 4 ang; it seems nothing is available for lower resolution than that. does this indicate salt (despite properties screening), which I thought would only show high resolution spots, and only a few, not as many as observed in the image. Essentially, could anyone offer any further analysis of the diffraction pattern and help me decide whether this is a protein or salt? Thanks in advance for all feedback!!! Cheers, Nick ________________________________________ Nicholas Noinaj University of Kentucky School of Medicine Department of Molecular and Cellular Biochemistry Biomedical Biological Sciences Research Building, Rm 236 741 S. Limestone Lexington, Ky 40536 Lab: 859-323-8183 Home: 859-228-0978 [EMAIL PROTECTED] www.noinaj.com
