Depends on which antibody you're using. Different manufacturers tend to light up different stuff. We very rarely see just one band - typically it's a bunch of weak bands as well as smears.
How strong is the band? Can you cut it out of the gel and do a tryptic digest and MS identification? If the band is strong, consider the possibility that you've somehow contaminated your strain with some other expressor... Artem -----Original Message----- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Raji Edayathumangalam Sent: Friday, April 20, 2007 4:36 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Ecoli protein staining with AntiHis? Hi Everyone, I see a band that lights up in my anti-His Western blots. While I investigate what else the band might be (truncated protein etc.), does anyone know whether there is an E. coli protein that migrates ~ 30-40kDa (SDS-PAGE), which binds to anti-His antibodies? Thanks. Raji
