Hi all i m trying to solve a structure at 2.2A using MIR. i obtained phases using solve. density modification n model building was carried out using phenix. i m solving structure in p212121 space group, with 4 fold NCS. i got almost 80% model built using phenix, rest of the model was built using coot. Right now i am refining the structure at 2.2 Presently Rfree is 36.31% n Rcrys is 35.77%.
Now whatever changes i made, Rfree continues to decrease while Rcryst is more or less stable. what could be the possible reasons for such a condition. it would be a great help if i can get few tips to overcome this problem. Thanx in advance Vineet Gaur
