Dear All: I am refining a protein-peptide complex struture at 2.6 angstrom resolution. The data was obtain from a co-crystal and the wilson B factor of the data is about 70. The affinity between protein and peptide is about 10E-7 to 10E-8 molar. Protein fragment of the structure has a common B facor about 50. But surprisingly, the average B factor of the peptide is as high as 130, although the peptide can be clearly traced from the the electron density map. All residues of the peptide have such a high B factor. My question is how can I reduce the abnormal high B factor to a common level or if this high B factor acceptable. And another question is if this high B fator will influence the final refiment level.
Thanks. -- Jiamu Du State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences (CAS)