Dear all, I am having a problem with refining my structure using refmac5. Basically, I am trying to make some small changes to several places on the backbone. But the thing is that, after restrained refinement, the changes I made would be all gone and the chain stayed exactly where they were before I made those changes. I'm using Turbo for building the model. The resolution I'm working on now is 1.2 A. The refmac was working fine when I was working at a lower resolution (2 A) and I was able to rebuild a whole helix and refine successfully with refmac. Right now, I am still able to change rotamers of single residues and refmac will keep these type of changes for me. It is only when changes are made on the backbones that refmac will refine the structure back to what it was before the changes were made. Other than refining to highest resolution 1.2 A, I'm pretty much using all the default parameters from the restrained refinement, except that, under Geometric parameters, I changed all the overall wt restraints from 1.0 to 2.0. Weighing term has been varied from 0.3 to 0.5 and no difference was made. I guess it's probably some small mistakes I made while running the refmac, but I can't figure out what it is. Can anyone help me out real quick? Thanks! Regards, Xiaofei
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