at around 4 molar P-buffer freezes clean. Best wishes
Kornelius On Fri, 22 Jun 2007 14:42:00 +0200 Claudia Scotti <[EMAIL PROTECTED]> wrote: > Sorry: forgotten the pH: the crystals grow between pH 6.5 > and 6.7. > > Claudia > > > Claudia Scotti > Dipartimento di Medicina Sperimentale > Sezione di Patologia Generale > Universita' di Pavia > Piazza Botta, 10 > 27100 Pavia > Italia > Tel. 0039 0382 986335/8/1 > Facs 0039 0382 303673 > > ---------------------------------------- > > Date: Fri, 22 Jun 2007 14:15:47 +0200 > > From: [EMAIL PROTECTED] > > Subject: [ccp4bb] How to replace 2.45M K/Na phosphate > buffer > > To: CCP4BB@JISCMAIL.AC.UK > > > > Dear list, > > > > I've crystals in a condition including 2,45 M K/Na > phosphate buffer for the well condition, and 1 microl > protein + 0.8 microl Na/K phosphate buffer + 0.25 microl > 30% 1,6-hexanediol in the drop condition. > > > > These crystals dissolve in several cryoprotectant > solutions and survive only in malonic acid, with which > they give diffraction at 7-8 A. > > > > Among the several different possibilities, I'd like to > try to optimize the crystals, and one possiblity among > others would be to change the buffer. > > > > Any suggestions, please, about how to replace K/Na > phosphate? At these concentrations it is acting as a > buffer but also as a precipitant and I was wondering if > anybody has experienced nice buffer-salt combinations > that proved to be useful starting from a similar > condition. > > > > Thanks a lot, > > > > Claudia > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > Claudia Scotti > > Dipartimento di Medicina Sperimentale > > Sezione di Patologia Generale > > Universita' di Pavia > > Piazza Botta, 10 > > 27100 Pavia > > Italia > > Tel. 0039 0382 986335/8/1 > > Facs 0039 0382 303673 > > > _________________________________________________________________ > > Make every IM count. Download Windows Live Messenger > and join the i’m Initiative now. It’s free. > > > http://im.live.com/messenger/im/home/?source=TAGWL_June07 > > _________________________________________________________________ > Make every IM count. Download Windows Live Messenger and > join the i’m Initiative now. It’s free. > http://im.live.com/messenger/im/home/?source=TAGWL_June07 > ---------------------------------------------- Kornelius Zeth Max Planck Institute for Developmental Biology Dept. Protein Evolution Spemannstr. 35 72076 Tuebingen, Germany [EMAIL PROTECTED] Tel -49 7071 601 323 Fax -49 7071 601 349
