There are four molecules in the asymmetric unit. And this density is present in every molecules.
-----Original Message----- From: ANA MARIA MISIC [mailto:[EMAIL PROTECTED] Sent: Thursday, July 26, 2007 5:24 PM To: Wu, Mousheng Subject: Re: [ccp4bb] unknown density in structure Hi Mousheng - Did you check to see if that falls on a special position? That can lead to a buildup of electron density, and the symmetrical shape is suspect. good luck Ana M. Misic Research Assistant Department of Biomolecular Chemistry Department of Bacteriology University of Wisconsin - Madison 420 Henry Mall, Rm 230 Madison, WI 53706 Lab Phone: (608)265-9282 Fax: (608)262-9865 ----- Original Message ----- From: "Wu, Mousheng" <[EMAIL PROTECTED]> Date: Thursday, July 26, 2007 4:21 pm Subject: [ccp4bb] unknown density in structure To: CCP4BB@JISCMAIL.AC.UK > Hi, everyone, > > > > I am recently solving a structure at 1.8A. After I fitted all residues > into the model, I found that there was an extra density in a pocket > consisted of E, N, R and other hydrophobic residues. The density is > strong and connected like a butterfly. I checked the crystallization > condition. I can not figure out what it is. It seems that it is not from > the crystallization components. > > > > Does anyone know how to figure out what the density is? Is there any > program or database I can use to predict the potential ligand? > > Thanks. > > > > Mousheng >
