I guess this is the old fashioned way, but it seems simpler to me! There are 2 strands labelled T
I find it easier to run a program than use coot. distang xyzin 65*pdb RADI CA 3 end This shows you that A 85 -ish is linked to T 11-23 with symop X-1/2,-Y+3/2,-Z and B ??? is linked to T 41-53 with symop X-1,Y,Z Then I run pdbset xyzin 65*pdb SYMGEN X-1/2,-Y+3/2,-Z end And that gives me the symmetry equivalent of T 11-23 which I want. With an editor I cut out those residues, and replace the ones in the original file. Then repeat the exercise with pdbset xyzin 65*pdb SYMGEN X-1,Y,Z end and cut and replace T 41-53 Eleanor (PS - use COOT though to check that the new coordinate file exactly overlaps the old one! A typing error is a disaster.. bc_panlf wrote: > Hi all: > I am a beginner for protein crystallography. After I used Arp/wrap to > build a model structure from molecular replacement, one part of the output > model structure is very strange, the first beta strand is isolated and not > fit to the other part. someone tell me coot can do this ,somehow > symmetrically translate. but i don't know how.can anyone tell me how to > fix this problem? > i post the pdb and mtz file in the attachment. > Thank you very much !! >
