Hi Bernhard,
Type: centrosymmetric rubredoxin
in google and you'll get a few papers including what I think was the
original work by Zawadzke & Berg.
Bart
Zawadzke LE, Berg JM.
Related Articles, Links
Abstract The structure of a centrosymmetric protein crystal.
Proteins. 1993 Jul;16(3):301-5.
Crystals of racemic rubredoxin, prepared by independent chemical
synthesis of the two enantiomers, have been grown and characterized. The
unit cell contains two molecules, one of each enantiomer. Examination of
the intensity distribution in the diffraction pattern revealed that the
crystals are centrosymmetric. This was confirmed by solution of the
structure to 2 A resolution via molecular replacement methods. The
electron density maps are of very high quality due to the fact that the
phase of each reflection must be exactly 0 degrees or exactly 180
degrees. These results demonstrate the feasibility of using synthetic
racemic proteins to yield centrosymmetric protein crystals with electron
density maps that have very low phase error and model bias.
Bernhard Rupp wrote:
Dear All,
there was a paper (quite) a while ago where someone made
for the first time a racemic protein mixture, obtained a
centrosymmetric structure and solved it (not the 2003
PNAS paper by the Eisenberg grp).
Hints appreciated.
Thx, br
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