[ccp4bb] off-topic-Heat shock protein gets precipitated

[shivesh kumar]

Dear all
I am trying to purify HSP from C.trachomatis,a 57kDa protein for
crystallization.The yield is very good.The problem is autodegradation and
precipitation.I never reached to 1.5mg/ml concentration.There is a His-tag
at C-ter.I appreciate all the suggestins regarding get rid of at least
precipitation.Also appreciate worth suggestions from groups working with
chaperonins.Thanx in advance.
Shivesh kumar