Hi all i m solving a structure at 2.2 A using MIR. rt now my Rfree is 30.55% and Rcryst is 28.51%. i have already carried out B factor refinement n water has also been picked up to 2.3 sigma cut off. now i m not able to refine the structure any further.
The protein has been purified directly from the source. At this pt of refinement i m able to see lot of unaccounted densities, far bigger to b accounted by water or sugar, as we don't have any information abt the possible molecules that can interact with this protein. . is this unaccounted density be the reason why i m not able to refine the strucure? thanx in advance Vineet Gaur
