I second Dr. Loll's question, and would like to be CC'd in whatever MS tips,
including
service-providers, are sent. I have been having a bit of a debacle with a
certain MS service provider.
Jacob Keller
==============Original message text===============
On Wed, 05 Sep 2007 11:41:52 am CDT Patrick Loll wrote:
I wonder if anyone would care to share experiences/ideas/biases that
relate to the use of mass spectrometry to verify the identity of
protein constructs used for crystallization. Our experience with
different MS facilities has been checquered.
Specifically:
What's the current thinking on the best approach to get masses for
intact proteins of moderate size (say, 40 kD)? ESI-TOF?
What kind of resolution should one hope to obtain in such cases
(10E-04?)
Any suggestions as to good facilities offering fee for service MS
characterization are welcome (but should be shared off line, I think;
continental US only).
Thanks,
Pat
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Patrick J. Loll, Ph. D. (215) 762-7706
Associate Professor FAX: (215) 762-4452
Department of Biochemistry & Molecular Biology
Director, Biochemistry Graduate Program
Drexel University College of Medicine
Room 10-102 New College Building
245 N. 15th St., Mailstop 497
Philadelphia, PA 19102-1192 USA
[EMAIL PROTECTED]
===========End of original message text===========
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Jacob Keller
Northwestern University
6541 N. Francisco #3
Chicago IL 60645
(847)491-2438
[EMAIL PROTECTED]
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