Hello all, I am forwarding this email as requested by a lab member due to technical difficulties in school mail accounts.
Ray >Hello, > >I usually try out Hampton crystal screen I & II, and PEG/ion screens >on my proteins, sometimes varying protein concentrations and >incubation temperature. If no hints are obtained, I will go back to >test alternative constructs and purification protocols. With more >and more companies selling crystal screens, and the accumulation of >knowledge from structural genomics projects, I worry that my >practice might have been outdated. I wonder if people could share >with me: > >1) What is your favorite set of crystal screens that maximize the >chance of getting the crystals while minimize the sample usage, and >perhaps more importantly, when do you decide to stop for better >constructs? > >2) Are there any other screens targeting nucleic acid and NA-protein >complexes other than the Natrix and the Nucleic Acid Mini Screen >sold by Hampton Research? Here I mean real different conditions, not >just a re-label. > >I'd appreciate your thoughts. > >Best >
