Dear Herman: It is due to the occupancy obviously. Previously, someone mensioned that in this resolution it is not suitable to refine the occupancy. So I think it is better for me to keep the occupancy to 1. Do you have some case for this situation in the PDB bank?
Thanks. On 11/1/07, [EMAIL PROTECTED] < [EMAIL PROTECTED]> wrote: > > Deart Jamu, > You probably do not have full occupancy. Set the occupancy of the > inhibitor to say 0.6 and refine again and see what happens to the > B-factors. > Herman > > ** > ------------------------------ > *From:* CCP4 bulletin board [mailto:[EMAIL PROTECTED] *On Behalf Of *Jiamu > Du > *Sent:* Thursday, November 01, 2007 1:05 PM > *To:* CCP4BB@JISCMAIL.AC.UK > *Subject:* [ccp4bb] the B facot of soaked substrate > > > Dear All: > I am refining a protein structure with a soaked substrate. > The resolution is 2.5 A. The B factor of protein is around 40, while the B > factor of the soaked substrate is as high as 80. > The density looks fine. > Is this structure acceptable? Or is there anyone who can give me an > example of this situation in the PDB bank? > Thanks. > > > -- > Jiamu Du > State Key Laboratory of Molecular Biology > Institute of Biochemistry and Cell Biology Shanghai Institutes for > Biological Sciences > Chinese Academy of Sciences (CAS) > -- Jiamu Du State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences (CAS)
