Simon, We routinely obtain structures from protein solutions with a big pellet of ligand in the bottom of the tube. For co-crystallizations we add 1mM compound to a 0.3mM solution of the protein and incubate overnight. Many of the compounds are only soluble to 50micromolar, so we get a lot of precipitate. The next day, we spin the tube at high speed, and use the supernatant for crystallization trials. We have started from 100mM stocks in 100% DMSO or ethanol. This has worked for compounds ranging for picomolar to micromolar affinity, which surprised us, but it worked.
Regards, Kendall On 12/11/07 11:55 AM, "Yue Li" <[EMAIL PROTECTED]> wrote: > Hi all, > > I have one ligand which is insoluble in water, and I would like to > co-crystallize it with my protein. Is there any other method except for > dissolving it in DMSO ? > > Thanks > > Simon
