[ccp4bb] Codon Optimized Expression

[Looney, Andrea Lynn (Andrea Hevrdeys)]

Dear All,
 
I am getting very little (not zero) expression of my protein.  I am curious to 
know if it is worthwhile to codon optimize my gene, which is ~1200bp,  for 
E.coli.  Can you have "too much of a good thing"?  Can codon optimizing 
overwhelm the cell or in some way cause death or lowered expression?
 
Thank you all,
 
Andrea L. Hevrdeys