Hi All, Maybe, I should not have asked this question:Can anybody give me the hints (or point to the references) on the impact of His tag on crystallization experiments. In perticular:
1, With or without His tag, which one is better for crystallization?2, If I successfully crystallized N-terminal, what information can I get to assist my set-up of C-terminal crystallization set-up.
Sorry for taking up your time because of my inadequate knowledge Thank you for your help Yanming