from the horses mouth ..
----- Forwarded message from [EMAIL PROTECTED] -----
Date: Mon, 03 Mar 2008 10:16:41 +0000
From: Alexander Golovanov <[EMAIL PROTECTED]>
Reply-To: Alexander Golovanov <[EMAIL PROTECTED]>
Subject: Re: Fwd: Re: [ccp4bb] Glu+Arg solutions for solubility
To: Stephen Prince <[EMAIL PROTECTED]>
Don't use salts, as it says (otherwise you will have to titrate pH a
lot to get it back to neutral). Just L-Arg and L-Glu. First, put
L-Arginine powder in some water, start dissolving it, then add L-Glu
powder, stir them together, top up the volume as required. They are
only soluble together at 1 M. The final pH of the 1M stock will be
(by itself) around 6.4 or close to neutral, in our experience.
good luck!
Sasha
At 09:17 03/03/2008, you wrote:
... from a bulletin board I subscribe to, are there any tricks for
making the stock (pH?) ...
On Thu, February 28, 2008 4:33 pm, Paul Paukstelis wrote:
posting for a colleague:
In Golovanov et al. 2004 they indicate it is possible to make a 1M
solution of free amino acids (not amino acid salts) Glu+Arg in the
context of generating solutions for keeping proteins soluble. Has anyone
been able to do this?
----- End forwarded message -----
______________________________________________________________________
S.M. Prince Phone: +44 (0)161 306 8919
Faculty of Life Sciences FAX: +44 (0)161 306 5201
Manchester Interdisciplinary Biocentre
University of Manchester
131 Princess Street e-mail: [EMAIL PROTECTED]
Manchester
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______________________________________________________________________
--
Dr Alexander 'Sasha' Golovanov
Lecturer
Faculty of Life Sciences and
Manchester Interdisciplinary Biocentre
The University of Manchester
131 Princess Street
Manchester M1 7DN, UK
Tel. No: +44 (0) 161 306 5813 Office (G.024)
+44 (0) 161 306 5812 NMR room
FAX: +44 (0) 161 306 5201
e-mail [EMAIL PROTECTED]
web: http://www.mib.manchester.ac.uk/aboutus/people/details.aspx?val=97941
----- End forwarded message -----
______________________________________________________________________
S.M. Prince Phone: +44 (0)161 306 8919
Faculty of Life Sciences FAX: +44 (0)161 306 5201
Manchester Interdisciplinary Biocentre
University of Manchester
131 Princess Street e-mail: [EMAIL PROTECTED]
Manchester
M1 7DN
U.K.
______________________________________________________________________
