Touche'
Jacob
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Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: [EMAIL PROTECTED]
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----- Original Message -----
From: "Artem Evdokimov" <[EMAIL PROTECTED]>
To: "'Jacob Keller'" <[EMAIL PROTECTED]>;
<CCP4BB@JISCMAIL.AC.UK>
Sent: Monday, March 03, 2008 6:31 PM
Subject: RE: [ccp4bb] Copper Staining
There's an even faster reagent for staining - takes abut 1 minute overall
and the results look just like dear old Coomassie. This reagent works
wonderfully for us. Yes, the recipe is proprietary - but if you add up the
cost of reagents for normal Coomassie and compare - this one isn't much
more
expensive.
http://www.novexin.com/products/InstantBlue.html
Artem
P.S. I am not affiliated with Novexin in any way shape or form, but their
gel stain does kick butt.
-----Original Message-----
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of
Jacob
Keller
Sent: Monday, March 03, 2008 6:03 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Copper Staining
Dear Crystallographers:
just to share something I found out recently, that is really helpful to
know:
5 min Copper Stain protocol
Normal SDS-PAGE gels can be stained in 5 min with 300mM CuCl2. Simply
remove
the gel, rinse ~10s in water, place 5 min in CuCl2, and the gel is
negatively stained, with protein bands appearing clear, and background
opaque white. To visualize, I put it on a flatbed scanner in a darkened
room. It works in my hands every bit as well as coomassie (at least as
sensitive, if not more), and if you want, you can stain afterwards in
coomassie with the usual effect. To destain, place gel in EDTA. The
original
paper is Lee et al, Anal. Biochem. 1987. Instead of waiting hours, you can
see the results in 5 min with copper! Also, there is no smell, and it can
be
re-used, to a point. Gels are stable in H2O for months, say Lee et al.
Jacob
p.s. I am not in copper comodities...
p.p.s. if anybody knows of down-sides to this, please let me know.
