Hi Chen, In this case, it seems that linker region is of great importance for the proper folding of the two linked domains. I have not much experience in linker region design, generally use (GS)5-10 times. However it depends on individual case. Anyone who has successful experience in linker region design might share your success with others. Thanks a lot.
Regards, Donghui On 3/5/08, Daniel Jin <[EMAIL PROTECTED]> wrote: > > Hi, > > I have a protein with two independently folded domains. I can express > either one in bacteria with pretty good expression yield. However, when I > put them together with a linker, the expression drops significantly. I can > barely see any soluble protein and most of it is now inclusion bodies. I > used the same bacteria strain and expression/purification conditions. Is it > normal? Any suggestion about how to improve? Many thanks. > > Best, > Chen > > > > ------------------------------ > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it > now.<http://us.rd.yahoo.com/evt=51733/*http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ> > >
