Crystals are supposed to be red if you have oxidized iron in the heme and deep pink/scarlet if the iron is reduced. If you removed the iron (for example EDTA during purification) or substituted with something during purification or crystallization, you could loose the color. It is trivial to check your crystals on the synchrotron beamline with fluorescence spectrum and "see" which metals are there. Cheers, Nukri
Ruslan Sanishvili (Nukri), Ph.D. GM/CA-CAT, Bld. 436, D007 Biosciences Division, ANL 9700 S. Cass Ave. Argonne, IL 60439 Tel: (630)252-0665 Fax: (630)252-0667 [EMAIL PROTECTED] <mailto:[EMAIL PROTECTED]> ________________________________ From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Jan Schoepe Sent: Wednesday, March 12, 2008 2:49 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Color of heme containing Xtals Hello everybody, I wonder if anybody has experience with heme (or to be more precise: heme b) containing proteins which Xtals do not look red under the microscope. How might the technique for crystallization (e.g. sitting drop, hanging drop) influence the intensity of the color? Many thanks! Jan ________________________________ Lesen Sie Ihre E-Mails jetzt einfach von unterwegs. <http://uk.rd.yahoo.com/evt=51524/*http://de.mobile.yahoo.com/interstiti al?refer=e00127> .
