Hello All,
I'm trying to back-FT an EM map to produce Fs and phases to use in
molecular replacement, but am having a problem with running SFALL.
Here's the issue: When running the back-transform in SFALL (generating
structure factors from a map), I get the following error:
Incorrect sampling grid factors 255 255 255
The map parameters are given below. Does anyone have any idea of how
to deal with this?
Thanks,
Larry
Number of columns, rows, sections ............... 256 256 256
Map mode ........................................ 2
Start and stop points on columns, rows, sections -128 127
-128 127 -128 127
Grid sampling on x, y, z ........................ 255 255 255
Cell dimensions ................................. 459.00000
459.00000 459.00000 90.00000 90.00000 90.00000
Fast, medium, slow axes ......................... X Y Z
Minimum density ................................. -1.24222
Maximum density ................................. 2.25668
Mean density .................................... 0.06370
Rms deviation from mean density ................. 0.00000
Space-group ..................................... 1
Number of titles ................................ 3
--
Lawrence Shapiro, Ph.D.
Associate Professor
Dept. of Biochemistry and Molecular Biophysics
Jules and Doris Stein Professor of Research to Prevent Blindness
Edward S. Harkness Eye Institute
Columbia University
701 West 168th Street, 7th Floor
New York, NY 10032
Tel: (212)342-6029
Fax: (212)342-6026
e-mail: [EMAIL PROTECTED]
[EMAIL PROTECTED]
http://www.shapirolab.org
