Dear Colleague,
At the Grenoble Outstation of the EMBL we have been developing a
humidity control device (HC1) used to improve the diffraction quality of
crystals. This device can be easily placed on a home source setup or a
synchrotron beamline. It has been tested with numerous crystals and the
results so far are very promising. In order to further asses the
capabilities of the new device we need to test as many proteins as
possible. This is why we would like to offer the opportunity of testing
your crystals with this new dehydration device.
So if...
- you have poorly diffracting crystals that are close to useful resolution
- you would like to push your good crystals to even higher resolution
- your crystals do not cryo-protect well but diffract at room temperature
- your crystals change space group upon binding compounds or due to
environmental changes
- you have a high solvent content and/or areas of loose packing in your
crystal
- or you have managed to improve your crystals by dehydrating but it is
difficult to monitor and/or reproduce the results
… then the HC1 may be just what you need.
We are looking for people who will share some of their protein or
crystals in order for us to test them with the HC1 at the ESRF. We will
apply for beam time and perform dehydration experiments. If a suitable
crystal is found then it will be frozen and a dataset will be collected
for you.
In order to evaluate the chance of success with the new humidity control
system we need certain information. This information will be
confidential. The more information we have the better the chances of
finding a suitable test crystal. Please complete a submission form in
either MS Office 03 or PDF format. Feel free to add any extra
information you think is useful. For help see additional notes at the
end of the documents. The documents can be downloaded from:
http://www.embl-grenoble.fr/groups/instr/index.html
Once you have filled in the questionnaire either save it or convert it
to PDF and send it to [EMAIL PROTECTED] If you find it easier, fill it in
by hand, and then send it to the following address. Please feel free to
contact me if you require any further information.
--
Juan Sanchez-WEATHERBY
Tel: 33(0)47620 7266
Website:
http://www.embl.fr
http://www.embl-grenoble.fr/groups/instr/index.html
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Postal address:
EMBL Grenoble
6 rue Jules Horowitz, BP 181
38042 Grenoble Cedex 9
FRANCE
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