Hi
If you mean organic small molecules, then the opinion for the last 15
years at least is probably "yes, unless you know you'll have a phase
change".
Most small molecule crystals don't have the same problems with
needing cryoprotectants as macromolecules, due in large part to not
having a large proportion of water in the lattice, so the process is
somewhat more straightforward. Also, most small molecule crystals can
be handled quite happily in the absence of mother liquor, and you
don't have to worry about them drying out while transferring to the
fibre (rather than loop) which would normally be used for mounting
them. Of course, there are numerous exceptions to the "most" I'm
referring to here.
In most cases you'll get a substantially better structure at cryo
temperatures (of course, what "better" means may be open to debate).
On 19 Jun 2008, at 09:47, Jayashankar wrote:
Dear Scientists and Friends,
I am not sure, whether organic crystals need to be in cryo stream
necessarily during data collection from an in house
xray machine .
How most of the organic crystals have been solved mostly?
--
S.Jayashankar
(A bit confused new generation researcher).
Research Student
Institute for Biophysical Chemistry
Hannover Medical School
Germany
Harry
--
Dr Harry Powell, MRC Laboratory of Molecular Biology, MRC Centre,
Hills Road, Cambridge, CB2 2QH
