Dear CCP4BBers,
One of those questions regarding purification rather than
crystallography:
Reading the Qiagen manual for the Ni-NTA matrices, in the table of
compatibility of reagents with Ni-NTA matrices, SDS is mentioned
(only together with sarkosyl) as "Not recommended, but up to 0.3% has
been used successfully in some cases". Google is failing me to find
those cases, since pretty much every paper mentioning Ni binding also
mentions SDS-PAGE. Also, arginine is mentioned just as "not
recommended".
Why are SDS and arginine "not recommended", what are the physical and
chemical underlying problems?
Can they be used at low concentration without damaging the matrix or
abolishing binding?
Which are the maximal concentrations people had success with?
Thanks a lot,
Jose Antonio Cuesta Seijo
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Jose Antonio Cuesta-Seijo
Cancer Genomics and Proteomics
Ontario Cancer Institute, UHN
MaRS TMDT Room 4-902M
101 College Street
M5G 1L7 Toronto, ON, Canada
Phone: (416)581-7544
Fax: (416)581-7562
email: [EMAIL PROTECTED]
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