Hi everybody,
we have solved the X-ray structure of a GFP (green fluorescent protein) containing complex. During refinement of the structure we now encountered the problem of connecting the GFP chromophore covalently to the polypeptide chain. We introduced the chromophore to our model as a separate ligand with separate toplogy. So far we could not find a way to integrate it in the primary sequence. Any idea how this problem can be solved? Which programs might be useful? Thank you very much in advance. Best, Sheng & Axel ************************************************************************* Dr. Sheng CUI 崔胜 Department of Chemistry and Biochemistry Gene Center University of Munich Feodor-Lynen-Str. 25 81377 Munich, Germany Tel: +49 89 2180-76984 Fax: +49 89 2180-76999 e-mail: <mailto:[EMAIL PROTECTED]> [EMAIL PROTECTED] Web: <http://www.lmb.uni-muenchen.de/hopfner/welcome.html> http://www.lmb.uni-muenchen.de/hopfner/welcome.html ***************************************************************************
