6M Guanidiniumhydrochloride works pretty good, the question is only
will you be able to refold it correctly ?
I believe a better approach is to avoid precipitation in the first
place and optimize your purification procedure in such a way that it
works. When do you observe precipitate ? Dialysis, then us e a fast
desalting column instead.
Jürgen
On 1 Oct 2008, at 06:12, ANDY DODDS wrote:
Hello,
following on from a previous topic about precipitating protein, but I
believe a distinct caveat of this warranting a separate thread, I
would like to know people's experiences in trying to get precipitated
protein back into solution? Is there a way or are there many ways?
Any experiences would be welcome,
yours,
Andy
-
Jürgen Bosch
University of Washington
Dept. of Biochemistry, K-426
1705 NE Pacific Street
Seattle, WA 98195
Box 357742
Phone: +1-206-616-4510
FAX: +1-206-685-7002
Web: http://faculty.washington.edu/jbosch
